{"product_id":"proteintech-12442-2-ap","title":"Proteintech, 12442-2-AP, PDE1A Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PDE1A (12442-2-AP) by Proteintech is a Polyclonal antibody targeting PDE1A in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n12442-2-AP targets PDE1A in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, human brain tissue,  rat brain tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPDE1A(Calcium\/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A) is also named as Cam-PDE 1A, 61 kDa Cam-PDE, hCam-1 and belongs to the cyclic nucleotide phosphodiesterase (PDEs) family. The PDEs play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and\/or cGMP to their respective nucleoside 5-prime monophosphates. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 57 kDa to 63 kDa. There is a report showing the major 67-kD PDE1A isoform identified in human sperm associates tightly with calmodulin and is not activated by Ca(2+)\/calmodulin(PMID:12135876).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3157 Product name: Recombinant human PDE1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 185-545 aa of BC022480 Sequence: TRYDLINRFKIPVSCLITFAEALEVGYSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNILINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGIDRAKTMSLILHAADISHPAKSWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKAETSSYVASSSTTIVGLHIADALRRSNTKGSMSDGSYSPDYSLAAVDLKSFKNNLVDIIQQNKERWKELAAQGESDLHKNSEDLVNAEEKHDETHS Predict reactive species\nFull Name: phosphodiesterase 1A, calmodulin-dependent\nCalculated Molecular Weight: 545 aa, 61 kDa\nObserved Molecular Weight: 61 kDa\nGenBank Accession Number: BC022480\nGene Symbol: PDE1A\nGene ID (NCBI): 5136\nRRID: AB_2162893\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P54750\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868971651241,"sku":"12442-2-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-12442-2-ap","provider":"Iright","version":"1.0","type":"link"}