{"product_id":"proteintech-12574-1-ap","title":"Proteintech, 12574-1-AP, TSP50 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TSP50 (12574-1-AP) by Proteintech is a Polyclonal antibody targeting TSP50 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n12574-1-AP targets TSP50 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF7 cells,  HEK-293 cells,  human testis tissue,  MCF-7 cells,  mouse testis tissue\nPositive IHC detected in: human ovary tumor tissue,  human breast cancer tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2400\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway(PMID:21385156). There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage(PMID:11782390). The full length protein has a signal peptide with 39 amino acids and two glycosylation sites.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3247 Product name: Recombinant human TSP50 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 32-385 aa of BC033016 Sequence: LRSAGCWGAGEAPGALSTADPADQSVQCVPKATCPSSRPRLLWQTPTTQTLPSTTMETQFPVSEGKVDPYRSCGFSYEQDPTLRDPEAVARRWPWMVSVRANGTHICAGTIIASQWVLTVAHCLIWRDVIYSVRVGSPWIDQMTQTASDVPVLQVIMHSRYRAQRFWSWVGQANDIGLLKLKQELKYSNYVRPICLPGTDYVLKDHSRCTVTGWGLSKADGMWPQFRTIQEKEVIILNNKECDNFYHNFTKIPTLVQIIKSQMMCAEDTHREKFCYELTGEPLVCSMEGTWYLVGLVSWGAGCQKSEAPPIYLQVSSYQHWIWDCLNGQALALPAPSRTLLLALPLPLSLLAAL Predict reactive species\nFull Name: testes-specific protease 50\nCalculated Molecular Weight: 385 aa, 43 kDa\nObserved Molecular Weight: 50-55 kDa\nGenBank Accession Number: BC033016\nGene Symbol: TSP50\nGene ID (NCBI): 29122\nRRID: AB_10646475\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UI38\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869388918953,"sku":"12574-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-12574-1-ap","provider":"Iright","version":"1.0","type":"link"}