{"product_id":"proteintech-12712-1-ap","title":"Proteintech, 12712-1-AP, GCNF Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GCNF (12712-1-AP) by Proteintech is a Polyclonal antibody targeting GCNF in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n12712-1-AP targets GCNF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: PC-3 cells,  K-562 cells,  mouse testis tissue\nPositive IP detected in: mouse testis tissue\nPositive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNR6A1 (Nuclear Receptor Subfamily 6 Group A member), also known as  GCNF (Germ Cell Nuclear Factor), is an orphan member of the nuclear receptor gene superfamily. It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation. Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death. GCNF has been shown to be a repressor of OCT-4 and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis. This antibody specifically reacts with the 55kd NR6A1 protein.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3395 Product name: Recombinant human NR6A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC030600 Sequence: MERDEPPPSGGGGGGGSAGFLEPPAALPPPPRNGFCQDELAELDPGTNDRAEQRTCLICGDRATGLHYGIISCEGCKGFFKRSICNKRVYRCSRDKNCVMSRKQRNRCQYCRLLKCLQMGMNRKAIREDGMPGGRNKSIGPVQISEEEIERIMSGQEFEEEANHWSNHGDSDHSSPGNRASESNQPSPGSTLSSRSVELNGFMAFREQYMGMSVPPHYQYIPHLFSYSGHSPLLPQQARSLDPQSYSLIHQLLSAEDLEPLGTPMLIEDGYAVTQAELFALLCRLADELLFRQIAWIKKLPFFCELSIKDYTCLLSSTWQELILLSSLTVYSKQIFGELADVTAKYSPSD Predict reactive species\nFull Name: nuclear receptor subfamily 6, group A, member 1\nCalculated Molecular Weight: 475 aa, 54 kDa\nObserved Molecular Weight: 54 kDa\nGenBank Accession Number: BC030600\nGene Symbol: GCNF\nGene ID (NCBI): 2649\nRRID: AB_10640439\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q15406\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868998193321,"sku":"12712-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-12712-1-ap","provider":"Iright","version":"1.0","type":"link"}