{"product_id":"proteintech-13043-1-ap","title":"Proteintech, 13043-1-AP, MCM4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MCM4 (13043-1-AP) by Proteintech is a Polyclonal antibody targeting MCM4 in WB, IHC, IF-P, IP, ELISA applications with reactivity to human samples\n13043-1-AP targets MCM4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells,  HEK-293 cells,  HL-60 cells,  human liver tissue\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human colon cancer tissue, mouse testis tissue,  rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse testis tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of \ntwo neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box \nof the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of MCM4 protein.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3703 Product name: Recombinant human MCM4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 515-859 aa of BC031061 Sequence: SKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKT Predict reactive species\nFull Name: minichromosome maintenance complex component 4\nCalculated Molecular Weight: 863 aa, 97 kDa\nObserved Molecular Weight: 97 kDa\nGenBank Accession Number: BC031061\nGene Symbol: MCM4\nGene ID (NCBI): 4173\nRRID: AB_2142293\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P33991\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868894089385,"sku":"13043-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13043-1-ap","provider":"Iright","version":"1.0","type":"link"}