{"product_id":"proteintech-13189-1-ap","title":"Proteintech, 13189-1-AP, RAB25 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RAB25 (13189-1-AP) by Proteintech is a Polyclonal antibody targeting RAB25 in WB, IHC, ELISA applications with reactivity to human samples\n13189-1-AP targets RAB25 in WB, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HT-29 cells, Caco-2 cells,  T-47D cells\nPositive IHC detected in: human renal cell carcinoma tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRAB25 (RAB25, member RAS oncogene family), also known as RAB11C or CATX-8, is a small GTPase belonging to the RAS superfamily of proteins. RAB25 is a key regulator of receptor recycling and polarized vesicular trafficking in epithelial cells. Unlike many RAB proteins, RAB25 expression is predominantly restricted to epithelial tissues and is frequently dysregulated in carcinomas. It functions as a critical determinant of cellular fate through its context-dependent roles in modulating major signaling pathways, including integrin recycling, growth factor receptor trafficking (e.g., EGFR, IGF1R), and autophagy.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3864 Product name: Recombinant human RAB25 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-213 aa of BC033322 Sequence: MGNGTEEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFSHDSRTTIGVEFSTRTVMLGTAAVKAQIWDTAGLERYRAITSAYYRGAVGALLVFDLTKHQTYAVVERWLKELYDHAEATIVVMLVGNKSDLSQAREVPTEEARMFAENNGLLFLETSALDSTNVELAFETVLKEIFAKVSKQRQNSIRTNAITLGSAQAGQEPGPGEKRACCISL Predict reactive species\nFull Name: RAB25, member RAS oncogene family\nCalculated Molecular Weight: 213 aa, 23 kDa\nObserved Molecular Weight: 24 kDa\nGenBank Accession Number: BC033322\nGene Symbol: RAB25\nGene ID (NCBI): 57111\nRRID: AB_2877921\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P57735\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869492170921,"sku":"13189-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13189-1-ap","provider":"Iright","version":"1.0","type":"link"}