{"product_id":"proteintech-13214-1-ap","title":"Proteintech, 13214-1-AP, RPGRIP1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RPGRIP1 (13214-1-AP) by Proteintech is a Polyclonal antibody targeting RPGRIP1 in WB, IHC, ELISA applications with reactivity to human, mouse samples\n13214-1-AP targets RPGRIP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Y79 cells, HEK-293 cells,  HL-60 cells\nPositive IHC detected in: mouse eye tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRPGRIP1 is a photoreceptor protein that interacts with retinitis pigmentosa GTPase regulator protein and is a key component of cone and rod photoreceptor cells. The biological functions of RPGRIP1 are complex. Mutations in this gene lead to autosomal recessive congenital blindness. In the eye, it is expressed in amacrine neurons and photoreceptors, and in numerous other tissues, albeit at greatly reduced levels. Moreover, it exists multiple isoforms with species-specific subcellular localization patterns, and the different isoforms perform cell-specific functions. Within photoreceptors, RPGRIP1 localizes predominantly to outer segments (OS) of rods and cones in humans, whereas in bovine eyes, it is found only in rod OS.  In contrast, in mice, RPGRIP1 is found primarily in connecting cilia (CC), where it localizes between the ciliary axoneme and the plasma membrane. RPGRIP1 has multiple isoforms with MW 147 kDa, 70-76 kDa, 103 kDa, and 66 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4089 Product name: Recombinant human RPGRIP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 320-612 aa of BC039089 Sequence: EKEHQVVSYSRRKHGKRIGVQGKNRMEYLSLNILNGNTPQQVNYTEWKFSETNSFIGDGFKNQHEEEEMTLSHSALKQKEPLHPVNDKESSEQGSEVSEAQTTDSDDVIVPPMSQKYPKADSEKMCIEIVSLAFYPEAEVMSDENIKQVYVEYKFYDLPLSETETPVSLRKPRAGEEIHFHFSKVIDLDPQEQQGRRRFLFDMLNGQDPDQGHLKFTVVSDPLDEEKKECEEVGYAYLQLWQILESGRDILEQELDIVSPEDLATPIGRLKVSLQAAAVLHAIYKEMTEDLFS Predict reactive species\nFull Name: retinitis pigmentosa GTPase regulator interacting protein 1\nCalculated Molecular Weight: 147 kDa\nObserved Molecular Weight: 70 kDa\nGenBank Accession Number: BC039089\nGene Symbol: RPGRIP1\nGene ID (NCBI): 57096\nRRID: AB_10639367\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96KN7\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869757657257,"sku":"13214-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13214-1-ap","provider":"Iright","version":"1.0","type":"link"}