{"product_id":"proteintech-13349-1-ap","title":"Proteintech, 13349-1-AP, FBLIM1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe FBLIM1 (13349-1-AP) by Proteintech is a Polyclonal antibody targeting FBLIM1 in IHC, ELISA applications with reactivity to human samples\n13349-1-AP targets FBLIM1 in IF, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFBLIM1, also known as CAL, Mig-2-interacting protein or Migfilin, is a cytoplasmic protein that belongs to the LIM superfamily. FBLIM1 is a protein found in cell-cell and cell-ECM connections where it co-localizes with FLNA\/C and FLNB. FBLIM1 was found to bind directly to FLNA\/C and to be an important regulator of cell shape and motility. FBLIM1 exerts its influence on cellular functions by interacting with various binding partners; FLN via its N- terminal domain, VASP and Src via its proline-rich region, and kindlin-2 and the cardiac transcription factor, CSX\/NKX2-5 via its C-terminal LIM domains. Three isoforms exist for FBLIM1 due to alternative splicing events, namely FBLP-1A, FBLP-1 and FBLP-1B. FBLIM1 serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. It is associated with actin stress fiber at cell-ECM focal adhesion sites. FBLP-1A and FBLP-1B are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. FBLP-1 is localized at actin stress fibers. FBLIM1 is implicated in cell shape modulation (spreading) and motility. FBLIM1 participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. It may also regulate the assembly of filamin-containing signaling complexes that control actin assembly. In addition, FBLIM1 is capable of translocating to the nucleus and regulating gene expression. This antibody is a rabbit polyclonal antibody raised against full length human FBLIM1 antigen.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3907 Product name: Recombinant human FBLIM1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-373 aa of BC019895 Sequence: MASKPEKRVASSVFITLAPPRRDVAVAEEVRQAVCEARRGRPWEAPAPMKTPEAGLAGRPSPWTTPGRAAATVPAAPMQLFNGGCPPPPPVLDGEDVLPDLDLLPPPPPPPPVLLPSEEEAPAPMGASLIADLEQLHLSPPPPPPQAPAEGPSVQPGPLRPMEEELPPPPAEPVEKGASTDICAFCHKTVFPRELAVEAMKRQYHAQCFTCRTCRRQLAGQSFYQKDGRPLCEPCYQDTLERCGKCGEVVRDHIIRALGQAFHPSCFTCVTCARCIGDESFALGSQNEVYCLDDFYRKFAPVCSICENPIIPRDGKDAFKIECMGRNFHENCYRCEDCRILLSVEPTDQGCYPLNNHLFCKPCHVKRSAAGCC Predict reactive species\nFull Name: filamin binding LIM protein 1\nCalculated Molecular Weight: 373 aa, 41 kDa\nObserved Molecular Weight: 45-48 kDa\nGenBank Accession Number: BC019895\nGene Symbol: FBLIM1\nGene ID (NCBI): 54751\nRRID: AB_2262903\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8WUP2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869682487465,"sku":"13349-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13349-1-ap","provider":"Iright","version":"1.0","type":"link"}