{"product_id":"proteintech-13379-1-ap","title":"Proteintech, 13379-1-AP, Amphiphysin Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Amphiphysin (13379-1-AP) by Proteintech is a Polyclonal antibody targeting Amphiphysin in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n13379-1-AP targets Amphiphysin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue,  mouse lung tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: mouse brain tissue, human pancreas cancer tissue,  mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAmphiphysin (AMPH), a synaptic vesicle-associated protein that is highly concentrated in nerve terminal, is an autoantigen of Stiff-Man syndrome with breast cancer. Amphiphysin contains a N-terminal BAR (Bin1\/Amphiphysin\/Rv167) domain followed by an endocytosis domain, and a C-terminal SH3 domain. Amphiphysin has been proposed to function as a linker between the clathrin coat and dynamin in the endocytosis of synaptic vesicles. It may also participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. (PMID: 8552632; 8245793; 10559861)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4204 Product name: Recombinant human AMPH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 346-695 aa of BC034376 Sequence: KKEETLLDLDFDPFKPEVTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQPQDTSLFTMQTDQSMICNLAESEQAPPTEPKAEEPLAAVTPAVGLDLGMDTRAEEPVEEAVIIPGADADAAVGTLVSAAEGAPGEEAEAETATVPAGEGVSLEEAKIGTETTEGAESAQPEAEELEATVPQEKVIPSVVIEPASNHEEEGENEITIGAEPKETTEDAAPPGPTSETPELATEQKPIQDPQPTPSAPAMGAADQLASAREASQELPPGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLD Predict reactive species\nFull Name: amphiphysin\nCalculated Molecular Weight: 695 aa, 76 kDa\nObserved Molecular Weight: 115-125 kDa\nGenBank Accession Number: BC034376\nGene Symbol: AMPH\nGene ID (NCBI): 273\nRRID: AB_2226789\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P49418\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868895924393,"sku":"13379-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13379-1-ap","provider":"Iright","version":"1.0","type":"link"}