{"product_id":"proteintech-13384-1-ap","title":"Proteintech, 13384-1-AP, AP3B1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe AP3B1 (13384-1-AP) by Proteintech is a Polyclonal antibody targeting AP3B1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n13384-1-AP targets AP3B1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, mouse thymus tissue,  COLO 320 cells,  HeLa cells,  HepG2 cells,  SKOV-3 cells\nPositive IP detected in: COLO 320 cells\nPositive IHC detected in: rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAP3B1 is the 140-kDa β3A subunit of the adaptor-related protein complex-3 (AP-3), a ubiquitous heterotetrameric complex that is localized to the trans-Golgi network and endosomes and is involved in protein trafficking to lysosomes or specialized endosomal-lysosomal organelles (PMID: 9182526; 9545220). This complex is composed of two larger subunits (δ and β3A or β3B), a medium subunit (μ3A or μ3B), and a small subunit (σ3A or σ3B). The absence of the β3A subunit (AP3B1) results in the loss of stability of AP3 and leads to degradation of μ3A, to which β3A is directly bound, while the other subunits are variably affected (PMID: 16507770). AP3B1 contains three main domains: the N-terminal head domain, the hinge, and the C-terminal ear domain. It has been reported as a target of IP(7)-mediated pyrophosphorylation (PMID: 19934039). Defects in AP3B1 are the cause of Hermansky-Pudlak syndrome type 2 (HPS2) (PMID: 10024875; 16507770).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4225 Product name: Recombinant human AP3B1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 763-1094 aa of BC038444 Sequence: KTSDSSNDESSSIEDSSSDSESESEPESESESRRVTKEKEKKTKQDRTPLTKDVSLLDLDDFNPVSTPVALPTPALSPSLMADLEGLHLSTSSSVISVSTPAFVPTKTHVLLHRMSGKGLAAHYFFPRQPCIFGDKMVSIQITLNNTTDRKIENIHIGEKKLPIGMKMHVFNPIDSLEPEGSITVSMGIDFCDSTQTASFQLCTKDDCFNVNIQPPVGELLLPVAMSEKDFKKEQGVLTGMNETSAVIIAAPQNFTPSVIFQKVVNVANVGAVPSGQDNIHRFAAKTVHSGSLMLVTVELKEGSTAQLIINTEKTVIGSVLLRELKPVLSQG Predict reactive species\nFull Name: adaptor-related protein complex 3, beta 1 subunit\nCalculated Molecular Weight: 1094 aa, 121 kDa\nObserved Molecular Weight: 140 kDa\nGenBank Accession Number: BC038444\nGene Symbol: AP3B1\nGene ID (NCBI): 8546\nRRID: AB_2056499\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O00203\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868892876969,"sku":"13384-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13384-1-ap","provider":"Iright","version":"1.0","type":"link"}