{"product_id":"proteintech-13403-1-ap","title":"Proteintech, 13403-1-AP, KIF22 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe KIF22 (13403-1-AP) by Proteintech is a Polyclonal antibody targeting KIF22 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n13403-1-AP targets KIF22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, human heart tissue,  MKN-45 cells,  T-47D cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human stomach cancer tissue, human breast cancer tissue,  human brown disease,  human cervical cancer tissue,  mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nKIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4254 Product name: Recombinant human KIF22 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 4-353 aa of BC028155 Sequence: GGSTQQRRREMAAASAAAISGAGRCRLSKIGATRRPPPARVRVAVRLRPFVDGTAGASDPPCVRGMDSCSLEIANWRNHQETLKYQFDAFYGERSTQQDIYAGSVQPILRHLLEGQNASVLAYGPTGAGKTHTMLGSPEQPGVIPRALMDLLQLTREEGAEGRPWALSVTMSYLEIYQEKVLDLLDPASGDLVIREDCRGNILIPGLSQKPISSFADFERHFLPASRNRTVGATRLNQRSSRSHAVLLVKVDQRERLAPFRQREGKLYLIDLAGSEDNRRTGNKGLRLKESGAINTSLFVLGKVVDALNQGLPRVPYRDSKLTRLLQDSLGGSAHSILIANIAPERRFYL Predict reactive species\nFull Name: kinesin family member 22\nCalculated Molecular Weight: 665 aa, 73 kDa\nObserved Molecular Weight: 68-73 kDa\nGenBank Accession Number: BC028155\nGene Symbol: KIF22\nGene ID (NCBI): 3835\nRRID: AB_2249576\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q14807\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869414150313,"sku":"13403-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13403-1-ap","provider":"Iright","version":"1.0","type":"link"}