{"product_id":"proteintech-13578-1-ap","title":"Proteintech, 13578-1-AP, ABCG1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ABCG1 (13578-1-AP) by Proteintech is a Polyclonal antibody targeting ABCG1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n13578-1-AP targets ABCG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HUVEC cells, HeLa cells,  HepG2 cells,  mouse kidney tissue,  rat kidney tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: mouse placenta tissue, human tonsillitis tissue,  human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4479 Product name: Recombinant human ABCG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC029158 Sequence: MACLMAAFSVGTAMNASSYSAEMTEPKSVCVSVDEVVSSNMEATETDLLNGHLKKVDNNLTEAQRFSSLPRRAAVNIEFRDLSYSVPEGPWWRKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMKGAVLINGLPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLSCANTRTGSLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKGLAQGGRSIICTIHQPSAKLFELFDQLYVLSQGQCVYRGKVCNLVPYLRDLGLNCPTYHNPADFVMEVASGEYGDQNSRLVRAVREGMCDSDH Predict reactive species\nFull Name: ATP-binding cassette, sub-family G (WHITE), member 1\nCalculated Molecular Weight: 678 aa, 76 kDa\nObserved Molecular Weight: 75 kDa\nGenBank Accession Number: BC029158\nGene Symbol: ABCG1\nGene ID (NCBI): 9619\nRRID: AB_2220174\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P45844\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868837236905,"sku":"13578-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13578-1-ap","provider":"Iright","version":"1.0","type":"link"}