{"product_id":"proteintech-13700-1-ap","title":"Proteintech, 13700-1-AP, TBX21\/T-bet Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TBX21\/T-bet (13700-1-AP) by Proteintech is a Polyclonal antibody targeting TBX21\/T-bet in WB, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples\n13700-1-AP targets TBX21\/T-bet in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: NK-92 cells, A549 cells,  mouse thymus tissue,  RAW 264.7 cells\nPositive IP detected in: mouse thymus tissue\nPositive IF\/ICC detected in: NK-92 cells\nPositive FC (Intra) detected in: NK92\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4618 Product name: Recombinant human TBX21,T-bet protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC039739 Sequence: MGIVEPGCGDMLTGTEPMPGSDEGRAPGADPQHRYFYPEPGAQDADERRGGGSLGSPYPGGALVPAPPSRFLGAYAYPPRPQAAGFPGAGESFPPPADAEGYQPGEGYAAPDPRAGLYPGPREDYALPAGLEVSGKLRVALNNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKAEGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEPEAACNASNTHIFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENF Predict reactive species\nFull Name: T-box 21\nCalculated Molecular Weight: 535 aa, 58 kDa\nObserved Molecular Weight: 60-62 kDa\nGenBank Accession Number: BC039739\nGene Symbol: TBX21\/T-bet\nGene ID (NCBI): 30009\nENSEMBL Gene ID: ENSG00000073861\nRRID: AB_10666436\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UL17\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868853686441,"sku":"13700-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13700-1-ap","provider":"Iright","version":"1.0","type":"link"}