{"product_id":"proteintech-13860-1-ap","title":"Proteintech, 13860-1-AP, AEG-1\/MTDH Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe AEG-1\/MTDH (13860-1-AP) by Proteintech is a Polyclonal antibody targeting AEG-1\/MTDH in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n13860-1-AP targets AEG-1\/MTDH in WB, IHC, IF\/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells, A375 cells,  HeLa cells,  DU 145 cells,  HL-60 cells,  A549 cells,  mouse spleen tissue,  PC-3 cells,  HepG2 cells,  RAW 264.7 cells\nPositive IP detected in: MCF-7 cells\nPositive IHC detected in: human breast cancer tissue, mouse brain tissue,  human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4840 Product name: Recombinant human AEG-1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 233-582 aa of BC045642 Sequence: EQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKAEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET Predict reactive species\nFull Name: metadherin\nCalculated Molecular Weight: 582 aa, 64 kDa\nObserved Molecular Weight: 65-70 kDa\nGenBank Accession Number: BC045642\nGene Symbol: AEG-1\/MTDH\nGene ID (NCBI): 92140\nRRID: AB_2266734\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q86UE4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868838940841,"sku":"13860-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13860-1-ap","provider":"Iright","version":"1.0","type":"link"}