{"product_id":"proteintech-13930-1-ap","title":"Proteintech, 13930-1-AP, KIF3A Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe KIF3A (13930-1-AP) by Proteintech is a Polyclonal antibody targeting KIF3A in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse samples\n13930-1-AP targets KIF3A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, human cerebellum tissue,  mouse cerebellum tissue,  SH-SY5Y cells,  SK-N-SH cells\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: human gliomas tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse eye tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)-P: IF-P : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nKIF3A is a subunit of Kinesin II that is essential for intraflagellar transport (IFT). It functions as the anterograde motor to deliver ciliary components to the tip where the axoneme is assembled. Disruption of KIF3A has been reported to block mammalian ciliogenesis. Recently KIF3A has been found to localize to the mother centriole and is required for centrioles organization. This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-\/- lysate. (23386061)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4954 Product name: Recombinant human KIF3A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC045542 Sequence: MPINKSEKPESCDNVKVVVRCRPLNEREKSMCYKQAVSVDEMRGTITVHKTDSSNEPPKTFTFDTVFGPESKQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAIPELRGIIPNSFAHIFGHIAKAEGDTRFLVRVSYLGIYNEEVRDLLGKDQTQRLEVIERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGATNMNEHSSRSHAIFTITIECSEKGIDGNMHVRMGKLHLVDLAGSERQAKTGATGQRLKEATKINLSLSTLGNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKARINEDP Predict reactive species\nFull Name: kinesin family member 3A\nCalculated Molecular Weight: 80 kDa\nObserved Molecular Weight: 80 kDa\nGenBank Accession Number: BC045542\nGene Symbol: KIF3A\nGene ID (NCBI): 11127\nRRID: AB_2132063\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9Y496\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868882161833,"sku":"13930-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-13930-1-ap","provider":"Iright","version":"1.0","type":"link"}