{"product_id":"proteintech-14018-1-ap","title":"Proteintech, 14018-1-AP, BACH1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe BACH1 (14018-1-AP) by Proteintech is a Polyclonal antibody targeting BACH1 in WB, IHC, ELISA applications with reactivity to human samples\n14018-1-AP targets BACH1 in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, MCF-7 cells,  Raji cells,  HeLa cells,  Jurkat cells,  K-562 cells\nPositive IHC detected in: human cervical cancer tissue, human breast hyperplasia tissue,  human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunohistochemistry (IHC): IHC : 1:750-1:3000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBTB and CNC homology 1 (Bach1) isa transcription factor expressed in many tissues, with key roles in cell cycleregulation in homeostasis and in cancer in disease.What is the molecular weight of BACH1?Bach1 consists of736 amino acids and is amember of the cap'n'collar type of basic region leucine zipper factorfamily (CNC-bZip) with several atypical protein domains.Although its predicted MW is 82 kDa, it runsanomalouslyat100-110 kDa in SDS-PAGE.What is the cellular localization of BACH1?As it is a transcription factor, BACH1 is found in the nucleus of a number of mammalian cells, where it forms aheterodimer with small Maf proteins. Once it has formed part of a heterodimer, BACH1 is able to bind to theMafrecognition elements (MAREs)of target genes. During oxidative stress,BACH1 can be inactivated either byhemeor by cadmium, at which point it is exported from the nucleus into the cytoplasm, where it localizes tomicrotubules(PMID: 15809329).What is the function of BACH1?BACH1 regulates the production of reactive oxygen species, with many of its downstream targets such as hemeoxygenase-1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1) (PMID: 12356737; PMID: 15734732) involvedin theoxidative stress response. When the cell undergoes oxidative stress, BACH1 is displaced bynuclear factor(erythroid-derived2)-like-2 (Nrf2), as both compete in binding to the MAREs in target genes. BACH1 also plays arolein cellproliferation and survival, as it regulates several cell cycle genes such as cyclin-dependent kinase 6(CDK6) andBCL2-like 11 (BCL2L11).What is the role of BACH1 in disease?BACH1 has been described in a number of cancer types, including breast, prostate, and melanoma. The functionofBACH1 appears varied in these tissues, with evidence that it may both promote and repress metastasis,influenceepigenetics, and regulate the growth of cancer cells (PMID: 22875853; PMID: 28889753; PMID: 26787892).BACH1may act on the same gene in different contexts to repress or activate it (PMID: 8887638).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5128 Product name: Recombinant human BACH1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 387-736 aa of BC063307 Sequence: SSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLLKERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSFLISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQMSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE Predict reactive species\nFull Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1\nCalculated Molecular Weight: 82 kDa\nObserved Molecular Weight: 100-110 kDa\nGenBank Accession Number: BC063307\nGene Symbol: BACH1\nGene ID (NCBI): 571\nENSEMBL Gene ID: ENSG00000156273\nRRID: AB_2274498\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O14867\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868839956649,"sku":"14018-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-14018-1-ap","provider":"Iright","version":"1.0","type":"link"}