{"product_id":"proteintech-14276-1-ap","title":"Proteintech, 14276-1-AP, CNOT1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CNOT1 (14276-1-AP) by Proteintech is a Polyclonal antibody targeting CNOT1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n14276-1-AP targets CNOT1 in WB, IHC, IF\/ICC, IP, chIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human brain tissue, HEK293 cells,  mouse kidney tissue,  mouse spleen tissue,  mouse thymus tissue,  HEK-293 cells,  mouse brain tissue,  mouse heart tissue,  HeLa cells\nPositive IP detected in: mouse kidney tissue, HeLa cells\nPositive IHC detected in: human heart tissue, human spleen tissue,  human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HEK-293 cells, sodium arsenite treated HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms.This is a rabbit polyclonal antibody raised against part chain of C-terminal CNOT1 of human origin. CNOT1 exsits as many isoforms and molecular weight of isoforms are 267, 241 and 173 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5623 Product name: Recombinant human CNOT1 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-354 aa of BC040523 Sequence: MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNF Predict reactive species\nFull Name: CCR4-NOT transcription complex, subunit 1\nCalculated Molecular Weight: 267 kDa\nObserved Molecular Weight: 240-250 kDa, 174 kDa\nGenBank Accession Number: BC040523\nGene Symbol: CNOT1\nGene ID (NCBI): 23019\nRRID: AB_10888627\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: A5YKK6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868832420009,"sku":"14276-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-14276-1-ap","provider":"Iright","version":"1.0","type":"link"}