{"product_id":"proteintech-14283-1-ap","title":"Proteintech, 14283-1-AP, SFRP5 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SFRP5 (14283-1-AP) by Proteintech is a Polyclonal antibody targeting SFRP5 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n14283-1-AP targets SFRP5 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HUVEC cells, L02 cells,  mouse heart tissue,  mouse retina tissue\nPositive IHC detected in: human thyroid cancer tissue, human cervical cancer tissue,  mouse heart tissue,  rat eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A549 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSecreted frizzled-related protein 5 (SFRP5) is an anti-inflammatory adipokine whose expression is perturbed in models of obesity and type 2 diabetes. SFRP5 is secreted by adipocytes and that it controls the micro-environment of white adipose tissue under conditions of metabolic stress (PMID: 20558665). SFRP5 gene consists of 317 amino acid residues and 3 coding exons and maps to chromosome 10q24.1 in humans. SFRP5 was initially found to be highly expressed in retinal pigment epithelium cells and moderately expressed in the pancreas. SFRP5 is involved in the pathological process of obesity, insulin resistance, dyslipidemia, inflammation, and fibrosis and is associated with obesity, T2D, and ASCVD (PMID: 31199714, PMID: 32004418).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5555 Product name: Recombinant human SFRP5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC050435 Sequence: MRAAAAAGGVRTAALALLLGALHWAPARCEEYDYYGWQAEPLHGRSYSKPPQCLDIPADLPLCHTVGYKRMRLPNLLEHESLAEVKQQASSWLPLLAKRCHSDTQVFLCSLFAPVCLDRPIYPCRSLCEAVRAGCAPLMEAYGFPWPEMLHCHKFPLDNDLCIAVQFGHLPATAPPVTKICAQCEMEHSADGLMEQMCSSDFVVKMRIKEIKIENGDRKLIGAQKKKKLLKPGPLKRKDTKRLVLHMKNGAGCPCPQLDSLAGSFLVMGRKVDGQLLLMAVYRWDKKNKEMKFAVKFMFSYPCSLYYPFFYGAAEPH Predict reactive species\nFull Name: secreted frizzled-related protein 5\nCalculated Molecular Weight: 36 kDa\nObserved Molecular Weight: 36-42 kDa\nGenBank Accession Number: BC050435\nGene Symbol: SFRP5\nGene ID (NCBI): 6425\nRRID: AB_3085434\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q5T4F7\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887798571177,"sku":"14283-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-14283-1-ap","provider":"Iright","version":"1.0","type":"link"}