{"product_id":"proteintech-14418-1-ap","title":"Proteintech, 14418-1-AP, ATP1A1 Polyclonal antibody","description":"Size: 150ul\nThe ATP1A1 (14418-1-AP) by Proteintech is a Polyclonal antibody targeting ATP1A1 in WB, IHC, IF\/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications with reactivity to human, mouse, rat samples\n14418-1-AP targets ATP1A1 in WB, IHC, IF\/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: 37°C incubated HEK-293 cells, 37°C incubated mouse brain tissue,  37°C incubated HeLa cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human cervical cancer tissue, human colon tissue,  human liver cancer tissue,  human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human ovary tumor tissue, human cervical cancer tissue\nPositive IF\/ICC detected in: Caco-2 cells, HEK-293 cells\nPositive FC (Intra) detected in: HEK-293 cells\nPositive CoIP detected in: A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\nImmunofluorescence (IF)-P: IF-P : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:400-1:1600\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\nCo-Immunoprecipitation (CoIP): COIP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nATP1A1 is the catalytic component of Na+\/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig, chicken, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5763 Product name: Recombinant human ATP1A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 347-681 aa of BC050359 Sequence: TAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDI Predict reactive species\nFull Name: ATPase, Na+\/K+ transporting, alpha 1 polypeptide\nCalculated Molecular Weight: 113 kDa\nObserved Molecular Weight: 100-110 kDa\nGenBank Accession Number: BC050359\nGene Symbol: ATP1A1\nGene ID (NCBI): 476\nENSEMBL Gene ID: ENSG00000163399\nRRID: AB_2227873\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P05023\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868822229161,"sku":"14418-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-14418-1-ap","provider":"Iright","version":"1.0","type":"link"}