{"product_id":"proteintech-14495-1-ap","title":"Proteintech, 14495-1-AP, VTI1B Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe VTI1B (14495-1-AP) by Proteintech is a Polyclonal antibody targeting VTI1B in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n14495-1-AP targets VTI1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, C6 cells,  NIH\/3T3 cells,  human liver tissue,  HeLa cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human liver cancer tissue, human malignant melanoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFusion between membranes is mediated by specific SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp, which is part of several SNARE complexes in different transport steps (PMID: 12067063). Both proteins had a distinct but overlapping localization. VTI1A is localized predominantly in the TGN, VTI1B in late endosomes (PMID:12067063; 21262811). VTI1B forms a SNARE complex with STX7, STX8, and VAMP8, which functions in the homotypic fusion of late endosomes. It is a component of the SNARE complex composed of STX7, STX8, VAMP7, and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes. It has also been reported that VIT1B interacts with EpsinR, a protein involved in exocytic trafficking (PMID: 15371541).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5906 Product name: Recombinant human VTI1B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 13-232 aa of BC003142 Sequence: KLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEANETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLLLSIIILLELAILGGLVYYKFFRSH Predict reactive species\nFull Name: vesicle transport through interaction with t-SNAREs homolog 1B (yeast)\nCalculated Molecular Weight: 27 kDa\nObserved Molecular Weight: 29 kDa\nGenBank Accession Number: BC003142\nGene Symbol: VTI1B\nGene ID (NCBI): 10490\nRRID: AB_2216160\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UEU0\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869440331945,"sku":"14495-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-14495-1-ap","provider":"Iright","version":"1.0","type":"link"}