{"product_id":"proteintech-14634-1-ap","title":"Proteintech, 14634-1-AP, PPP1R15B Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PPP1R15B (14634-1-AP) by Proteintech is a Polyclonal antibody targeting PPP1R15B in WB, IF\/ICC, IP, ELISA applications with reactivity to human samples\n14634-1-AP targets PPP1R15B in WB, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A2780 cells, MCF-7 cells,  HEK-293 cells,  SGC-7901 cells,  MDA-MB-231 cells,  SKOV-3 cells\nPositive IP detected in: MCF-7 cells\nPositive IF\/ICC detected in: SH-SY5Y cells, HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nProtein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1\/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B  is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag6213 Product name: Recombinant human PPP1R15B;CReP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 369-713 aa of BC065280 Sequence: TTEVPLALEEESPSEGCPSSEIPMEKEPGEGRISVVDYSYLEGDLPISARPACSNKLIDYILGGASSDLETSSDPEGEDWDEEAEDDGFDSDSSLSDSDLEQDPEGLHLWNSFCSVDPYNPQNFTATIQTAARIVPEEPSDSEKDLSGKSDLENSSQSGSLPETPEHSSGEEDDWESSADEAESLKLWNSFCNSDDPYNPLNFKAPFQTSGENEKGCRDSETPSESIVAISECHTLLSCKVQLLGSQESECPDSVQRDVLSGGRHTHVKRKKVTFLEEVTEYYISGDEDRKGPWEEFARDGCRFQKRIQETEDAIGYCLTFEHRERMFNRLQGTCFKGLNVLKQC Predict reactive species\nFull Name: protein phosphatase 1, regulatory (inhibitor) subunit 15B\nCalculated Molecular Weight: 79 kDa\nObserved Molecular Weight: 100-110 kDa\nGenBank Accession Number: BC065280\nGene Symbol: PPP1R15B\nGene ID (NCBI): 84919\nRRID: AB_2300036\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q5SWA1\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868862042281,"sku":"14634-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-14634-1-ap","provider":"Iright","version":"1.0","type":"link"}