{"product_id":"proteintech-14768-1-ap","title":"Proteintech, 14768-1-AP, FEN1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe FEN1 (14768-1-AP) by Proteintech is a Polyclonal antibody targeting FEN1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n14768-1-AP targets FEN1 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, NIH\/3T3 cells\nPositive IP detected in: NIH\/3T3 cells\nPositive IHC detected in: human colon cancer tissue, human lung cancer tissue,  mouse ovary tissue,  mouse small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: NIH\/3T3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag6552 Product name: Recombinant human FEN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-380 aa of BC000323 Sequence: MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK Predict reactive species\nFull Name: flap structure-specific endonuclease 1\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 48 kDa\nGenBank Accession Number: BC000323\nGene Symbol: FEN1\nGene ID (NCBI): 2237\nRRID: AB_2102780\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P39748\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868901822633,"sku":"14768-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-14768-1-ap","provider":"Iright","version":"1.0","type":"link"}