{"product_id":"proteintech-14855-1-ap","title":"Proteintech, 14855-1-AP, RD3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RD3 (14855-1-AP) by Proteintech is a Polyclonal antibody targeting RD3 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n14855-1-AP targets RD3 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse retina tissue, rat retina tissue\nPositive IHC detected in: rat eye tissue, mouse eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: H9C2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRD3, or Retinal Degeneration 3, plays a critical role in regulating guanylate cyclase (GC) signaling and photoreceptor cell survival (PMID: 30559291). RD3 is highly conserved across vertebrates, with the human protein sharing high sequence identity with other primates and varying degrees of identity with other species (PMID: 29030614). The main functions of RD3 include inhibiting photoreceptor-specific guanylate cyclase activity and promoting the accumulation of retinal membrane guanylyl cyclase (RetGC) in the photoreceptor outer segment (PMID: 30559291). RD3 is essential for the normal expression of RetGC in photoreceptor cells and blocks RetGC catalytic activity. Mutations in the RD3 gene can lead to Leber congenital amaurosis type 12, which results in retinal degeneration. RD3 is also involved in the trafficking of RetGC from the endoplasmic reticulum to the photoreceptor outer segments, which is crucial for maintaining the normal function and survival of photoreceptors (PMID: 34537244).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag6641 Product name: Recombinant human RD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-195 aa of BC065541 Sequence: MSLISWLRWNEAPSRLSTRSPAEMVLETLMMELTGQMREAERQQRERSNAVRKVCTGVDYSWLASTPRSTYDLSPIERLQLEDVCVKIHPSYCGPAILRFRQLLAEQEPEVQEVSQLFRSVLQEVLERMKQEEEAHKLTRQWSLRPRGSLATFKTRARISPFASDIRTISEDVERDTPPPLRSWSMPEFRAPKAD Predict reactive species\nFull Name: retinal degeneration 3\nCalculated Molecular Weight: 22.7 kDa\nObserved Molecular Weight: 23 kDa\nGenBank Accession Number: BC065541\nGene Symbol: RD3\nGene ID (NCBI): 343035\nRRID: AB_3085443\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q7Z3Z2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887781466281,"sku":"14855-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-14855-1-ap","provider":"Iright","version":"1.0","type":"link"}