{"product_id":"proteintech-14995-1-ap","title":"Proteintech, 14995-1-AP, DDA1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe DDA1 (14995-1-AP) by Proteintech is a Polyclonal antibody targeting DDA1 in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples\n14995-1-AP targets DDA1 in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, mouse liver tissue,  HeLa cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:300-1:1200\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDDA1, also known as C19orf58 and PCIA1, belongs to the DDA1 family. DDA1 is an evolutionarily conserved gene located at 19p13.11 that may be involved in the activation of nuclear factor kappaB (NFκB). DDA1 encodes an 12 kDa protein with 102 AA residues whose orthologs share 82-92% identity with Arabidopsis, invertebrates, and vertebrates. Additionally, DDA1 might be involved in tumour formation and progression (PMID: 26942699, 28211159).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag6981 Product name: Recombinant human DDA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-102 aa of BC000615 Sequence: MADFLKGLPVYNKSNFSRFHADSVCKASNRRPSVYLPTREYPSEQIIVTEKTNILLRYLHQQWDKKNAAKKRDQEQVELEGESSAPPRKVARTDSPDMHEDT Predict reactive species\nFull Name: DET1 and DDB1 associated 1\nCalculated Molecular Weight: 12 kDa\nObserved Molecular Weight: 12 kDa\nGenBank Accession Number: BC000615\nGene Symbol: DDA1\nGene ID (NCBI): 79016\nRRID: AB_10859095\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9BW61\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868979187881,"sku":"14995-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-14995-1-ap","provider":"Iright","version":"1.0","type":"link"}