{"product_id":"proteintech-15095-1-ap","title":"Proteintech, 15095-1-AP, LGMN Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe LGMN (15095-1-AP) by Proteintech is a Polyclonal antibody targeting LGMN in WB, IHC, ELISA applications with reactivity to human, mouse samples\n15095-1-AP targets LGMN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: COLO 320 cells, HEK-293 cells,  HeLa cells,  mouse kidney tissue\nPositive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7170 Product name: Recombinant human LGMN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 82-433 aa of BC003061 Sequence: TPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY Predict reactive species\nFull Name: legumain\nCalculated Molecular Weight: 49 kDa\nObserved Molecular Weight: 36 kDa\nGenBank Accession Number: BC003061\nGene Symbol: LGMN\nGene ID (NCBI): 5641\nRRID: AB_3669198\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q99538\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887703281833,"sku":"15095-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-15095-1-ap","provider":"Iright","version":"1.0","type":"link"}