{"product_id":"proteintech-15169-1-ap","title":"Proteintech, 15169-1-AP, FEM1A Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe FEM1A (15169-1-AP) by Proteintech is a Polyclonal antibody targeting FEM1A in WB, IHC, ELISA applications with reactivity to human samples\n15169-1-AP targets FEM1A in WB, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: U-937 cells\nPositive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:200-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFEM1A, also named as Protein fem-1 homolog A-A, is a 654 amino acid protein, which Belongs to the fem-1 family. FEM1A is highly expressed in adult heart and skeletal muscle. FEM1A is a Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1A may participate in antiinflammatory signaling via its interaction with PTGER4.Furthermore, Fem1aexpression is down-regulated in rhabdomyosarcoma cells suggesting that the FEM1A gene may be involved in muscle differentiation or be at least a marker of abnormal differentiation in rhabdomyosarcoma. Knowing that a molecular weight of 76 kDa is predicted for human FEM1A, but phosphorylated FEM1A is 95-100 kDa (PMID: 19406122).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7357 Product name: Recombinant human FEM1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 322-669 aa of BC004988 Sequence: WRRAMELRHQGGEYLPKPEPPQLVLAYDYSREVNTTEELEALITDPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFERCIRLWKYALDMQQSNLEPLSPMTASSFLSFAELFSYVLQDRAAKGSLGTQIGFADLMGVLTKGVREVERALQLPREPGDSAQFTKALAIILHLLYLLEKVECTPSQEHLKHQTVYRLLKCAPRGKNGFTPLHMAVDKDTTNVGRYPVGRFPSLHVVKVLLDCGADPDSRDFDNNTPLHIAAQNNCPAIMNALIEAGAHMDATNAFKKTAYELLDEKLLARGTMQPFNYVTLQCLAARALDKNKIPYKGFIPEDLEAFIELH Predict reactive species\nFull Name: fem-1 homolog a (C. elegans)\nCalculated Molecular Weight: 74 kDa\nObserved Molecular Weight: 74 kDa\nGenBank Accession Number: BC004988\nGene Symbol: FEM1A\nGene ID (NCBI): 55527\nRRID: AB_2102618\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9BSK4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887638302889,"sku":"15169-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-15169-1-ap","provider":"Iright","version":"1.0","type":"link"}