{"product_id":"proteintech-15415-1-ap","title":"Proteintech, 15415-1-AP, BTF3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe BTF3 (15415-1-AP) by Proteintech is a Polyclonal antibody targeting BTF3 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n15415-1-AP targets BTF3 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, HT-29 cells,  HeLa cells,  HuH-7 cells,  RAW 264.7 cells\nPositive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBasic transcription factor 3 (BTF3), also known as nascent-polypeptide-associated complex (NAC) beta (NACB), belongs to the NAC-beta family. BTF3 serves as an oncogene and convey worse prognosis in gastric cancer, pancreatic cancer, osteosarcoma, cervical cancer, hypopharyngeal squamous cell carcinoma, prostate cancer , breast cancer. Due to alternative splicing, BTF3 is present in two different isoforms, BTF3a and BTF3b. BTF3a is the transcriptionally active form of BTF3, while the isoform lacking the first 44 amino acids of the BTF3a N-terminus, BTF3b, is transcriptionally inactive, two isoforms of BTF3, BTF3a and BTF3b corresponding to 23kDa and 18kDa(PMID: 23029305).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7701 Product name: Recombinant human BTF3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-160 aa of BC008062 Sequence: MKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNE Predict reactive species\nFull Name: basic transcription factor 3\nCalculated Molecular Weight: 22 kDa\nObserved Molecular Weight: 18 kDa, 23 kDa\nGenBank Accession Number: BC008062\nGene Symbol: BTF3\nGene ID (NCBI): 689\nRRID: AB_3669206\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P20290\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46885305286825,"sku":"15415-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-15415-1-ap","provider":"Iright","version":"1.0","type":"link"}