{"product_id":"proteintech-15518-1-ap","title":"Proteintech, 15518-1-AP, Beta Galactosidase Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Beta Galactosidase (15518-1-AP) by Proteintech is a Polyclonal antibody targeting Beta Galactosidase in WB, IHC, IF\/ICC, IF-P, ELISA applications with reactivity to human, mouse samples\n15518-1-AP targets Beta Galactosidase in WB, IHC, IF\/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: SH-SY5Y cells\nPositive IHC detected in: human prostate cancer tissue, human gliomas tissue,  human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human liver cancer tissue, human prostate cancer tissue,  mouse liver tissue\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nWhat is the molecular weight of β-galactosidase (GLB1)?The molecular weight of GLB1 is 76 kD, although this protein is known to have multiple isoforms with lowermolecular weight values.What is the function of GLB1?The common name of β-galactosidase is lactase. This enzyme is responsible for catalyzingthe hydrolysisoflactoseto monosaccharide glucose and galactose. GLB1 also catalyzes hydrolysis of polymericD-galactosylresidues (PMID: 23526073, doi: 10.1088\/1757-899x\/263\/2\/022046).How is GLB1 used in our food?GLB1 isolated from bacteria, fungi, yeast, plants, and other sources has been applied to the manufacturingof lactose-hydrolyzed products (PMID: 28500401).How is GLB1 part of a positive feedback loop?GLB1 transgalactosylates lactose, resulting in allolactose. Allolactose is the inducer for the lac operon andbinds to the lac repressor, prompting GLB1 synthesis (PMIDs: 23526073, 28500401, 26394634).What organisms express GLB1?GLB1 is ubiquitous in nature and is produced across kingdoms in plants, microorganisms, and animals.Properties, such as optimal pH range and temperature preference, vary between species; fungal GLB1prefers acidic conditions, while yeast and bacteria have optimal conditions at more neutral pH values(PMID: 28500401).How is GLB1 utilized in transgenic animals?Bacterial GLB1 is a widely used reporter gene in transgenic or knockout animals. ThelacZgene ofE. colihas been used to identifycis-acting DNA elements that play a key role for the regulation ofgene expressionin mice. ThelacZgene can be incorporated into an endogenous gene, and it is frequently a used to indicatethe success of Cre-loxP recombination events, which allows temporal control oflacZexpression.Mice apart of the Rosa26R line that also express Cre ubiquitously express Cre-induciblelacZ(PMID: 26394634).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7792 Product name: Recombinant human Beta galactosidase protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 329-677 aa of BC007493 Sequence: TSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV Predict reactive species\nFull Name: galactosidase, beta 1\nCalculated Molecular Weight: 76 kDa\nObserved Molecular Weight: 67 kDa\nGenBank Accession Number: BC007493\nGene Symbol: GLB1\/Beta-galactosidase\nGene ID (NCBI): 2720\nRRID: AB_2263448\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P16278\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868845854889,"sku":"15518-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-15518-1-ap","provider":"Iright","version":"1.0","type":"link"}