{"product_id":"proteintech-15647-1-ap","title":"Proteintech, 15647-1-AP, PDK2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PDK2 (15647-1-AP) by Proteintech is a Polyclonal antibody targeting PDK2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n15647-1-AP targets PDK2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human heart tissue, HeLa cells,  mouse skeletal muscle tissue,  NCI-H1299 cells,  rat heart tissue\nPositive IP detected in: mouse skeletal muscle tissue\nPositive IHC detected in: mouse brain tissue, human brain tissue,  human testis tissue,  human placenta tissue,  human kidney tissue,  human heart tissue,  human liver tissue,  human skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2400\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:100-1:600\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPDK2([Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial) is also named as PDHK2 and belongs to the PDK\/BCKDK protein kinase family. PDK2 plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDK2 is also involved in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. This protein has 2 isoforms produced by alternative splicing.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8183 Product name: Recombinant human PDK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 135-407 aa of BC005811 Sequence: LEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS Predict reactive species\nFull Name: pyruvate dehydrogenase kinase, isozyme 2\nCalculated Molecular Weight: 407 aa, 46 kDa\nObserved Molecular Weight: 46 kDa\nGenBank Accession Number: BC005811\nGene Symbol: PDK2\nGene ID (NCBI): 5164\nRRID: AB_2268006\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q15119\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868882620585,"sku":"15647-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-15647-1-ap","provider":"Iright","version":"1.0","type":"link"}