{"product_id":"proteintech-15704-1-ap","title":"Proteintech, 15704-1-AP, EIF4G1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EIF4G1 (15704-1-AP) by Proteintech is a Polyclonal antibody targeting EIF4G1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n15704-1-AP targets EIF4G1 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: BxPC-3 cells, HeLa cells,  HepG2 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells, Ethacrynic acid treated HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8342 Product name: Recombinant human EIF4G1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 296-645 aa of BC007788 Sequence: IEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN Predict reactive species\nFull Name: eukaryotic translation initiation factor 4 gamma, 1\nCalculated Molecular Weight: 1600 aa, 176 kDa\nObserved Molecular Weight: 230-250 kDa\nGenBank Accession Number: BC007788\nGene Symbol: EIF4G1\nGene ID (NCBI): 1981\nRRID: AB_2261979\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q04637\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868858863785,"sku":"15704-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-15704-1-ap","provider":"Iright","version":"1.0","type":"link"}