{"product_id":"proteintech-15713-1-ap","title":"Proteintech, 15713-1-AP, PIP5K1A Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PIP5K1A (15713-1-AP) by Proteintech is a Polyclonal antibody targeting PIP5K1A in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n15713-1-AP targets PIP5K1A in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, HeLa cells,  human heart tissue,  human liver tissue,  NCI-H1299 cells,  PC-12 cells\nPositive IP detected in: HeLa cells,  mouse heart tissue\nPositive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: Hela cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPhosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8340 Product name: Recombinant human PIP5K1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 151-500 aa of BC007833 Sequence: SLCSEPLIELCSSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPCVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTSTTLEKLEVAESEFTH Predict reactive species\nFull Name: phosphatidylinositol-4-phosphate 5-kinase, type I, alpha\nCalculated Molecular Weight: 56 kDa, 63 kDa\nObserved Molecular Weight: 63 kDa\nGenBank Accession Number: BC007833\nGene Symbol: PIP5K1A\nGene ID (NCBI): 8394\nRRID: AB_2164696\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q99755\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868920336553,"sku":"15713-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-15713-1-ap","provider":"Iright","version":"1.0","type":"link"}