{"product_id":"proteintech-15731-1-ap","title":"Proteintech, 15731-1-AP, NUF2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NUF2 (15731-1-AP) by Proteintech is a Polyclonal antibody targeting NUF2 in WB, IP, ELISA applications with reactivity to human samples\n15731-1-AP targets NUF2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HepG2 cells,  HeLa cells,  human liver tissue,  human testis tissue,  PC-3 cells,  human skeletal muscle tissue\nPositive IP detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. NUF2 was reported to be overexpressed in various kinds of carcinomas including lung cancer, cholangiocellular cancer, urinary bladder cancer, renal cell cancer, colorectal cancer, gastric cancer, ovarian cancer and serous adenocarcinoma. Elevated expression of NUF2 was associated with poorer prognosis of non-small cell lung cancer patients (PMID: 17079454). Knockdown of NUF2 significantly caused cell growth delay and increased apoptosis in cell lines of non-small cell lung cancer, colorectal cancer, gastric cancer and ovarian cancer.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8479 Product name: Recombinant human NUF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 105-464 aa of BC008489 Sequence: ETADILCPKAKRTSRFLSGIINFIHFREACRETYMEFLWQYKSSADKMQQLNAAHQEALMKLERLDSVPVEEQEEFKQLSDGIQELQQSLNQDFHQKTIVLQEGNSQKKSNISEKTKRLNELKLSVVSLKEIQESLKTKIVDSPEKLKNYKEKMKDTVQKLKNARQEVVEKYEIYGDSVDCLPSCQLEVQLYQKKIQDLSDNREKLASILKESLNLEDQIESDESELKKLKTEENSFKRLMIVKKEKLATAQFKINKKHEDVKQYKRTVIEDCNKVQEKRGAVYERVTTINQEIQKIKLGIQQLKDAAEREKLKSQEIFLNLKTALEKYHDGIEKAAEDSYAKIDEKTAELKRKMFKMST Predict reactive species\nFull Name: NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)\nCalculated Molecular Weight: 464 aa, 54 kDa\nObserved Molecular Weight: 50 kDa\nGenBank Accession Number: BC008489\nGene Symbol: NUF2\nGene ID (NCBI): 83540\nRRID: AB_2154586\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9BZD4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868958642345,"sku":"15731-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-15731-1-ap","provider":"Iright","version":"1.0","type":"link"}