{"product_id":"proteintech-15924-1-ap","title":"Proteintech, 15924-1-AP, RHEB Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RHEB (15924-1-AP) by Proteintech is a Polyclonal antibody targeting RHEB in WB, IHC, IF\/ICC, IF-P, ELISA applications with reactivity to human, mouse samples\n15924-1-AP targets RHEB in WB, IHC, IF\/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: DU 145 cells, human brain tissue,  HeLa cells,  3T3-L1 cells,  fetal human brain tissue\nPositive IHC detected in: human brain tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human pancreas cancer tissue\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine\/threonine kinase which augments protein synthesis.  Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8747 Product name: Recombinant human RHEB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC016155 Sequence: MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM Predict reactive species\nFull Name: Ras homolog enriched in brain\nCalculated Molecular Weight: 184 aa, 20 kDa\nObserved Molecular Weight: 15-20 kDa\nGenBank Accession Number: BC016155\nGene Symbol: RHEB\nGene ID (NCBI): 6009\nRRID: AB_2285084\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q15382\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868903624873,"sku":"15924-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-15924-1-ap","provider":"Iright","version":"1.0","type":"link"}