{"product_id":"proteintech-16479-1-ap","title":"Proteintech, 16479-1-AP, ADO Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ADO (16479-1-AP) by Proteintech is a Polyclonal antibody targeting ADO in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n16479-1-AP targets ADO in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: fetal human brain tissue, human brain tissue,  mouse spleen tissue\nPositive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nADO, or 2-aminoethanethiol dioxygenase, cysteamine dioxygenase, is a thiol dioxygenase in humans that adds two oxygen atoms to cysteamine, forming hypotaurine (PMID: 39637922). ADO has been reported to exhibit two distinct biological functions with a nonheme iron center. It catalyzes oxidation of both cysteamine in sulfur metabolism and N-terminal cysteine-containing proteins or peptides, such as regulator of G protein signaling 5 (RGS5) (PMID: 34508780). ADO is ubiquitously expressed with the highest levels in brain, heart, and skeletal muscle. Western blot analysis detected two bands at 28~30 kDa as indicated in publications (PMID: 17581819).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag9598 Product name: Recombinant human ADO protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-270 aa of BC018660 Sequence: MPRDNMASLIQRIARQACLTFRGSWGGRGASDRDAASGAEAPMQPGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPLPPNLPPVTYMHIYETDGFSLGVFLLKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGGGQRPRALPPEQQFEPPLQPREREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAPPYDPDDGRDCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP Predict reactive species\nFull Name: 2-aminoethanethiol (cysteamine) dioxygenase\nCalculated Molecular Weight: 270 aa, 30 kDa\nObserved Molecular Weight: 28 kDa-30 kDa\nGenBank Accession Number: BC018660\nGene Symbol: ADO\nGene ID (NCBI): 84890\nRRID: AB_2222204\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96SZ5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869443084457,"sku":"16479-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-16479-1-ap","provider":"Iright","version":"1.0","type":"link"}