{"product_id":"proteintech-16541-1-ap","title":"Proteintech, 16541-1-AP, AXIN1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe AXIN1 (16541-1-AP) by Proteintech is a Polyclonal antibody targeting AXIN1 in WB, IHC, ELISA applications with reactivity to human samples\n16541-1-AP targets AXIN1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HT-1080 cells,  HEK-293T cells\nPositive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAxis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Recent researches find that CircAXIN1 encodes a novel protein, AXIN1-295aa, which shows at around 40-55 kDa by Western Blot. AXIN1-295aa functions as an oncogenic protein, activating the Wnt signaling pathway to promote GC tumorigenesis and progression, suggesting a potential therapeutic target for GC. Proteintech's AXIN1 antibody 16541-1-AP is a rabbit polyclonal antibdy raised against the  N-terminus of human AXIN1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, rat, chicken\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag9858 Product name: Recombinant human AXIN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC044648 Sequence: MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKLARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQ Predict reactive species\nFull Name: axin 1\nCalculated Molecular Weight: 826aa,92 kDa; 862aa,95 kDa\nObserved Molecular Weight: 100-110 kDa\nGenBank Accession Number: BC044648\nGene Symbol: AXIN1\nGene ID (NCBI): 8312\nRRID: AB_2062409\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O15169\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868881047721,"sku":"16541-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-16541-1-ap","provider":"Iright","version":"1.0","type":"link"}