{"product_id":"proteintech-16617-1-ap","title":"Proteintech, 16617-1-AP, CAD Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CAD (16617-1-AP) by Proteintech is a Polyclonal antibody targeting CAD in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n16617-1-AP targets CAD in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human liver cancer tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides (PMID:25422319). CAD initiates and controls the flflux through the pathway and is regulated by allosteric effectors and phosphorylations through different signaling cascades (PMID:12438317). Moreover, CAD is found upregulated in tumors, while mutations that compromise its function are the cause of a severe infantile epileptic disease (PMID:36677714).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag9912 Product name: Recombinant human CAD protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 502-851 aa of BC014178 Sequence: VAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF Predict reactive species\nFull Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase\nCalculated Molecular Weight: 2225 aa, 243 kDa\nObserved Molecular Weight: 240 kDa\nGenBank Accession Number: BC014178\nGene Symbol: CAD\nGene ID (NCBI): 790\nRRID: AB_2878289\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P27708\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868910506153,"sku":"16617-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-16617-1-ap","provider":"Iright","version":"1.0","type":"link"}