{"product_id":"proteintech-16639-1-ap","title":"Proteintech, 16639-1-AP, EPLIN Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EPLIN (16639-1-AP) by Proteintech is a Polyclonal antibody targeting EPLIN in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n16639-1-AP targets EPLIN in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HUVEC cells, HEK-293 cells,  human placenta tissue\nPositive IP detected in: HepG2 cells, mouse liver tissue\nPositive IHC detected in: human prostate cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: PC-3 cells, HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. The 110 kDa EPLIN-β isoform represents the full-length protein and the 90 kDa EPLIN-α isoform lacks the amino-terminal 160 amino acids. EPLIN-a expression is often down-regulated in cancerous cells and tissues. (PMID: 10806352, 31644899)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag9994 Product name: Recombinant human LIMA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 261-401 aa of BC001247 Sequence: KPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAK Predict reactive species\nFull Name: LIM domain and actin binding 1\nCalculated Molecular Weight: 85 kDa\nObserved Molecular Weight: 90 kDa, 110 kDa\nGenBank Accession Number: BC001247\nGene Symbol: EPLIN\nGene ID (NCBI): 51474\nRRID: AB_2136657\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UHB6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868965654697,"sku":"16639-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-16639-1-ap","provider":"Iright","version":"1.0","type":"link"}