{"product_id":"proteintech-16675-1-ap","title":"Proteintech, 16675-1-AP, AMFR\/GP78 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe AMFR\/GP78 (16675-1-AP) by Proteintech is a Polyclonal antibody targeting AMFR\/GP78 in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n16675-1-AP targets AMFR\/GP78 in WB, IHC, IF\/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: 37°C incubated HepG2 cells, HepG2 cells\nPositive IP detected in: MCF-7 cells\nPositive IHC detected in: mouse ovary tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag10178 Product name: Recombinant human AMFR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 301-643 aa of BC069197 Sequence: LFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS Predict reactive species\nFull Name: autocrine motility factor receptor\nCalculated Molecular Weight: 643 aa, 73 kDa\nObserved Molecular Weight: 73 kDa\nGenBank Accession Number: BC069197\nGene Symbol: AMFR\/GP78\nGene ID (NCBI): 267\nRRID: AB_2226463\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UKV5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868849983657,"sku":"16675-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-16675-1-ap","provider":"Iright","version":"1.0","type":"link"}