{"product_id":"proteintech-16743-1-ap","title":"Proteintech, 16743-1-AP, PHKG1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PHKG1 (16743-1-AP) by Proteintech is a Polyclonal antibody targeting PHKG1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n16743-1-AP targets PHKG1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue\nPositive IP detected in: mouse skeletal muscle tissue\nPositive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:5000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPHKG1(Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform) is also named as phosphorylase kinase subunit gamma-1, PHKG and belongs to the CAMK Ser\/Thr protein kinase family. Phosphorylase kinase is a crucial glycogenolytic regulatory enzyme. The holoenzymes are composed of 16 subunit proteins containing equimolar ratios of 4 subunit types known as alpha, beta, gamma, and delta. This protein is the skeletal muscle isoform of phosphorylase kinase and kinetic differences exist between the cardiac and adult skeletal muscle enzymes, and the liver and skeletal muscle enzymes are known to be separate isozymes.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag10032 Product name: Recombinant human PHKG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-387 aa of BC051327 Sequence: MTRDEALPDSHSAQDFYENYEPKEILGRGVSSVVRRCIHKPTSQEYAVKVIDVTGGGSFSPEEVRELREATLKEVDILRKVSGHPNIIQLKDTYETNTFFFLVFDLMKRGELFDYLTEKVTLSEKETRKIMRALLEVICTLHKLNIVHRDLKPENILLDDNMNIKLTDFGFSCQLEPGERLREVCGTPSYLAPEIIECSMNEDHPGYGKEVDMWSTGVIMYTLLAGSPPFWHRKQMLMLRMIMSGNYQFGSPEWDDYSDTVKDLVSRFLVVQPQNRYTAEEALAHPFFQQYLVEEVRHFSPRGKFKVIALTVLASVRIYYQYRRVKPVTREIVIRDPYALRPLRRLIDAYAFRIYGHWVKKGQQQNRAALFENTPKAVLLSLAEEDY Predict reactive species\nFull Name: phosphorylase kinase, gamma 1 (muscle)\nCalculated Molecular Weight: 387 aa, 45 kDa\nObserved Molecular Weight: 40 kDa\nGenBank Accession Number: BC051327\nGene Symbol: PHKG1\nGene ID (NCBI): 5260\nRRID: AB_1851532\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q16816\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869571535017,"sku":"16743-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-16743-1-ap","provider":"Iright","version":"1.0","type":"link"}