{"product_id":"proteintech-16912-1-ap","title":"Proteintech, 16912-1-AP, CES1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CES1 (16912-1-AP) by Proteintech is a Polyclonal antibody targeting CES1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n16912-1-AP targets CES1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, Jurkat cells,  mouse liver tissue,  rat liver tissue\nPositive IHC detected in: human liver cancer tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:800-1:8000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCES1(liver carboxylesterase 1) is also named as SES1 and belongs to the type-B carboxylesterase\/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag10567 Product name: Recombinant human CES1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 215-566 aa of BC012418 Sequence: VTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL Predict reactive species\nFull Name: carboxylesterase 1 (monocyte\/macrophage serine esterase 1)\nCalculated Molecular Weight: 566 aa, 62 kDa\nObserved Molecular Weight: 60-63 kDa\nGenBank Accession Number: BC012418\nGene Symbol: CES1\nGene ID (NCBI): 1066\nRRID: AB_2077500\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P23141\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868905394345,"sku":"16912-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-16912-1-ap","provider":"Iright","version":"1.0","type":"link"}