{"product_id":"proteintech-17040-1-ap","title":"Proteintech, 17040-1-AP, METAP2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe METAP2 (17040-1-AP) by Proteintech is a Polyclonal antibody targeting METAP2 in WB, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n17040-1-AP targets METAP2 in WB, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, MCF-7 cells,  mouse pancreas tissue,  mouse testis tissue\nPositive IP detected in: MCF-7 cells\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMetAP2, also known as MAP2, P67EIF2 or p67, is one of the three known methionine aminopeptidases responsible for the removal of the N-terminal translation initiator methionine from newly synthesized proteins, which is a critical step in protein maturation (PMID: 27692546, 16568094). The enzyme binds two cobalt or manganese ions in its active site (PMID: 11524009, 12718546). As higher MetAP2 expression was observed in tumor (mesothelioma, neuroblastoma, and colorectal carcinoma) cells compared with normal cells, the enzyme serves as a target for anti-angiogenic compounds of natural origin, such as fumagillin and ovalicin (PMID: 11485930, 15927358, 15102683, 9224570). Selective inhibition of MetAP2 stops vascularization and tumor growth in animal models (PMID: 12118091). MetAP2 has 3 isoforms with molecular mass of 50-53 kDa and apparent molecular mass 67 kD.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag10564 Product name: Recombinant human METAP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 132-478 aa of BC013782 Sequence: GQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY Predict reactive species\nFull Name: methionyl aminopeptidase 2\nCalculated Molecular Weight: 478 aa, 53 kDa\nObserved Molecular Weight: 67 kDa\nGenBank Accession Number: BC013782\nGene Symbol: METAP2\nGene ID (NCBI): 10988\nRRID: AB_2144162\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P50579\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869709357225,"sku":"17040-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-17040-1-ap","provider":"Iright","version":"1.0","type":"link"}