{"product_id":"proteintech-17173-1-ap","title":"Proteintech, 17173-1-AP, BPGM Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe BPGM (17173-1-AP) by Proteintech is a Polyclonal antibody targeting BPGM in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n17173-1-AP targets BPGM in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293T cells, human plasma,  mouse liver tissue,  rat liver tissue,  HeLa cells,  Jurkat cells,  K-562 cells,  LNCaP cells,  U-87 MG cells,  mouse heart tissue,  mouse lung tissue\nPositive IP detected in: mouse liver tissue\nPositive IHC detected in: human liver cancer tissue,  human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:100-1:400\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBPGM is a cytosolic erythrocyte enzyme that controls intracellular levels of 2,3-bisphosphoglycerate, a key allosteric regulator of hemoglobin. By modulating hemoglobin oxygen affinity, BPGM ensures efficient oxygen delivery to tissues and supports physiological adaptation to hypoxia. Genetic disruption of BPGM leads to erythrocytosis and impaired oxygen release, underscoring its central role in red blood cell metabolic specialization. BPGM in HCC cells also induced both histone lactylation and M2 polarization of macrophages by lactate secretion. (PMID: 41514495)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag10731 Product name: Recombinant human BPGM protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-259 aa of BC017050 Sequence: MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK Predict reactive species\nFull Name: 2,3-bisphosphoglycerate mutase\nCalculated Molecular Weight: 259 aa, 30 kDa\nObserved Molecular Weight: 30 kDa\nGenBank Accession Number: BC017050\nGene Symbol: BPGM\nGene ID (NCBI): 669\nRRID: AB_2274991\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P07738\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868993507497,"sku":"17173-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-17173-1-ap","provider":"Iright","version":"1.0","type":"link"}