{"product_id":"proteintech-17270-1-ap","title":"Proteintech, 17270-1-AP, POLR2I Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe POLR2I (17270-1-AP) by Proteintech is a Polyclonal antibody targeting POLR2I in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n17270-1-AP targets POLR2I in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HEK-293 cells,  HepG2 cells\nPositive IHC detected in: mouse stomach tissue, rat liver tissue,  mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPOLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag11027 Product name: Recombinant human POLR2I protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-125 aa of BC017112 Sequence: MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE Predict reactive species\nFull Name: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa\nCalculated Molecular Weight: 125 aa, 15 kDa\nObserved Molecular Weight: 15 kDa\nGenBank Accession Number: BC017112\nGene Symbol: POLR2I\nGene ID (NCBI): 5438\nRRID: AB_10836936\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P36954\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869488664745,"sku":"17270-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-17270-1-ap","provider":"Iright","version":"1.0","type":"link"}