{"product_id":"proteintech-17737-1-ap","title":"Proteintech, 17737-1-AP, EDC4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EDC4 (17737-1-AP) by Proteintech is a Polyclonal antibody targeting EDC4 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human samples\n17737-1-AP targets EDC4 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HepG2 cells\nPositive IHC detected in: human liver cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:300-1:1200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEnhancer of mRNA-decapping protein 4 (EDC4) is a key regulator of mRNA decapping and degradation, playing a crucial role in the mRNA decay pathway. It is involved in the formation of processing bodies (P-bodies) in the cytoplasm, where it interacts with other decapping factors such as DCP1A and DCP2A to facilitate mRNA degradation (PMID: 16364915). EDC4 also interacts with the mammalian target of rapamycin complex 1 (mTORC1) and is involved in the regulation of immune system functions (PMID: 25514416; 25970328). Additionally, EDC4 has been shown to regulate DNA repair processes, with its deficiency leading to genome instability and hypersensitivity to DNA interstrand cross-linking drugs (PMID: 29511213).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag11784 Product name: Recombinant human EDC4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-388 aa of BC064567 Sequence: MASCASIDIEDATQHLRDILKLDRPAGGPSAESPRPSSAYNGDLNGLLVPDPLCSGDSTSANKTGLRTMPPINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAVSNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVNGKIQEEILVHIRQPEGTPLNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHSTWPVDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGYVKFWQIYIEGQDEPRCLHEWKPHDGRPLSCLLFCDNHKKQDPDVPFWRFLITGADQNRELKMWCTVSW Predict reactive species\nFull Name: enhancer of mRNA decapping 4\nCalculated Molecular Weight: 1401 aa, 152 kDa\nObserved Molecular Weight: 160 kDa\nGenBank Accession Number: BC064567\nGene Symbol: EDC4\nGene ID (NCBI): 23644\nRRID: AB_10665813\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q6P2E9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868901691561,"sku":"17737-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-17737-1-ap","provider":"Iright","version":"1.0","type":"link"}