{"product_id":"proteintech-17813-1-ap","title":"Proteintech, 17813-1-AP, SPRY1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SPRY1 (17813-1-AP) by Proteintech is a Polyclonal antibody targeting SPRY1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples\n17813-1-AP targets SPRY1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A2780 cells, HEK-293 cells,  HepG2 cells\nPositive IP detected in: HT-29 cells, A2780 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSprouty homolog 1 (SPRY1) belongs to the sprouty family. Spry-1 is a negative regulator of receptor tyrosine kinase signalling (PMID: 36543535). Spry1 and its related proteins have also been implicated as negative regulators of RhoA and its downstream effector Rho kinase through the non-canonical Wnt signaling (PMID: 19671746, PMID: 15184877). Sprouty-1 and Sprouty-2 proteins are expressed in early embryonic lung both in the epithelial cells of distal tubules and in peripheral mesenchymal cells (PMID: 22236546). Spry1 is a cytoplasmic protein that plays a critical role in kidney development and is primarily known to inhibit the Ras\/MEK\/ERK pathway (PMID: 21310958).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12129 Product name: Recombinant human SPRY1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-319 aa of BC063856 Sequence: MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPILSRSTSTGSAASSGSNSSASSEQGLLGRSPPTRPVPGHRSERAIRTQPKQLIVDDLKGSLKEDLTQHKFICEQCGKCKCGECTAPRTLPSCLACNRQCLCSAESMVEYGTCMCLVKGIFYHCSNDDEGDSYSDNPCSCSQSHCCSRYLCMGAMSLFLPCLLCYPPAKGCLKLCRRCYDWIHRPGCRCKNSNTVYCKLESCPSRGQGKPS Predict reactive species\nFull Name: sprouty homolog 1, antagonist of FGF signaling (Drosophila)\nCalculated Molecular Weight: 319 aa, 35 kDa\nObserved Molecular Weight: 35 kDa\nGenBank Accession Number: BC063856\nGene Symbol: SPRY1\nGene ID (NCBI): 10252\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O43609\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887815512233,"sku":"17813-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-17813-1-ap","provider":"Iright","version":"1.0","type":"link"}