{"product_id":"proteintech-17958-1-ap","title":"Proteintech, 17958-1-AP, LOX Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe LOX (17958-1-AP) by Proteintech is a Polyclonal antibody targeting LOX in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples\n17958-1-AP targets LOX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, MKN-45 cells,  SGC-7901 cells,  NIH\/3T3 cells,  mouse skeletal muscle tissue,  A375 cells\nPositive IP detected in: mouse skeletal muscle tissue\nPositive IHC detected in: human stomach cancer tissue, mouse eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLOX is a member of a multigene family (LOX, LOXL, LOXL2, LOXL3, and LOXL4). Lysyl oxidase (LOX), a copper-dependent enzyme, plays a critical role in the formation and repair of the extracellularmatrix (ECM) by catalyzing the crosslinking of elastin and collagen. LOX expression appears to be upregulated in malignancies and has recently been suggested to play a role in regulating tumor metastasis. It is synthesized as a 50 kDa proenzyme, secreted, and processed into an 32 kDa mature, active enzyme and an 18-kDa propeptide.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, rabbit\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12479 Product name: Recombinant human LOX protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-417 aa of BC074872 Sequence: MRFAWTVLLLGPLQLCALVHCAPPAAGQQQPPREPPAAPGAWRQQIQWENNGQVFSLLSLGSQYQPQRRRDPGAAVPGAANASAQQPRTPILLIRDNRTAAARTRTAGSSGVTAGRPRPTARHWFQAGYSTSRAREAGASRAENQTAPGEVPALSNLRPPSRVDGMVGDDPYNPYKYSDDNPYYNYYDTYERPRPGGRYRPGYGTGYFQYGLPDLVADPYYIQASTYVQKMSMYNLRCAAEENCLASTAYRADVRDYDHRVLLRFPQRVKNQGTSDFLPSRPRYSWEWHSCHQHYHSMDEFSHYDLLDANTQRRVAEGHKASFCLEDTSCDYGYHRRFACTAHTQGLSPGCYDTYGADIDCQWIDITDVKPGNYILKVSVNPSYLVPESDYTNNVVRCDIRYTGHHAYASGCTISPY Predict reactive species\nFull Name: lysyl oxidase\nCalculated Molecular Weight: 417 aa, 47 kDa\nObserved Molecular Weight: 32 kDa, 45-47 kDa\nGenBank Accession Number: BC074872\nGene Symbol: LOX\nGene ID (NCBI): 4015\nRRID: AB_2234541\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P28300\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868849197225,"sku":"17958-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-17958-1-ap","provider":"Iright","version":"1.0","type":"link"}