{"product_id":"proteintech-18135-1-ap","title":"Proteintech, 18135-1-AP, LYN Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe LYN (18135-1-AP) by Proteintech is a Polyclonal antibody targeting LYN in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human samples\n18135-1-AP targets LYN in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells,  HL-60 cells,  Jurkat cells,  K-562 cells,  L02 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human kidney tissue,  human hepatocirrhosis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12723 Product name: Recombinant human LYN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 132-512 aa of BC075002 Sequence: KDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDFDPVHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQADGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKRLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTREEPIYIITEYMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMTALSQGYRMPRVENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP Predict reactive species\nFull Name: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog\nCalculated Molecular Weight: 512 aa, 59 kDa\nObserved Molecular Weight: 53-56 kDa, 66-70 kDa\nGenBank Accession Number: BC075002\nGene Symbol: LYN\nGene ID (NCBI): 4067\nRRID: AB_11042319\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P07948\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869470544041,"sku":"18135-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-18135-1-ap","provider":"Iright","version":"1.0","type":"link"}