{"product_id":"proteintech-18139-1-ap","title":"Proteintech, 18139-1-AP, HYAL4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HYAL4 (18139-1-AP) by Proteintech is a Polyclonal antibody targeting HYAL4 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n18139-1-AP targets HYAL4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: K-562 cells, NIH\/3T3 cells,  mouse testis tissue,  rat testis tissue\nPositive IP detected in: mouse testis tissue\nPositive IHC detected in: mouse skin tissue, human placenta tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHyaluronic acid (HA), a glycosaminoglycan that is ubiquitously present in the extracellular space of higher animals, maintains matrix structure and controls cellular functions such as proliferation, differentiation, and locomotion. Hyal enzymes that catabolize HA, are involved in development and tumorigenesis.HYAL4, a member of the Hyal  family, is a chondroitin sulfate (CS)-specific endo-β-acetylgalactosaminidase. The expression of hHYAL4 mRNA is not ubiquitous but restricted to placenta, skeletal muscle, and testis (PMID: 23086929). This polyclonal antibody raised against 29-460aa of human HYAL4. The apparent molecular weight is larger than the calculated molecular weight of 54 kDa, which is likely due to posttranslational modification, presumably by glycosylation (PMID: 19889881).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12766 Product name: Recombinant human HYAL4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 29-460 aa of BC104788 Sequence: LKSISCLKPARLPIYQRKPFIAAWNAPTDQCLIKYNLRLNLKMFPVIGSPLAKARGQNVTIFYVNRLGYYPWYTSQGVPINGGLPQNISLQVHLEKADQDINYYIPAEDFSGLAVIDWEYWRPQWARNWNSKDVYRQKSRKLISDMGKNVSATDIEYLAKVTFEESAKAFMKETIKLGIKSRPKGLWGYYLYPDCHNYNVYAPNYSGSCPEDEVLRNNELSWLWNSSAALYPSIGVWKSLGDSENILRFSKFRVHESMRISTMTSHDYALPVFVYTRLGYRDEPLFFLSKQDLVSTIGESAALGAAGIVIWGDMNLTSSKANCTKVKQFVSSDLGSYIANVTRAAEVCSLHLCRNNGRCIRKMWNAPSYLHLNPASYHIEASEDGEFTVKGKASDTDLAVMADTFSCHCYQGYEGADCREIKTADGCSGVSP Predict reactive species\nFull Name: hyaluronoglucosaminidase 4\nCalculated Molecular Weight: 481 aa, 54 kDa\nObserved Molecular Weight: 60-65 kDa\nGenBank Accession Number: BC104788\nGene Symbol: HYAL4\nGene ID (NCBI): 23553\nRRID: AB_2878506\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q2M3T9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869696053417,"sku":"18139-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-18139-1-ap","provider":"Iright","version":"1.0","type":"link"}