{"product_id":"proteintech-18195-1-ap","title":"Proteintech, 18195-1-AP, LACTB Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe LACTB (18195-1-AP) by Proteintech is a Polyclonal antibody targeting LACTB in WB, IHC, IF-P, ELISA applications with reactivity to human samples\n18195-1-AP targets LACTB in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, human heart tissue,  HeLa cells\nPositive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse liver tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLACTB is also named as MRPL56,which is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form. This antibody is specific to LACTB.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12233 Product name: Recombinant human LACTB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 73-373 aa of BC067288 Sequence: DPEASPLAEPPQEQSLAPWSPQTPAPPCSRCFARAIESSRDLLHRIKDEVGAPGIVVGVSVDGKEVWSEGLGYADVENRVPCKPETVMRIASISKSLTMVALAKLWEAGKLDLDIPVQHYVPEFPEKEYEGEKVSVTTRLLISHLSGIRHYEKDIKKVKEEKAYKALKMMKENVAFEQEKEGKSNEKNDFTKFKTEQENEAKCRNSKPGKKKNDFEQGELYLREKFENSIESLRLFKNDPLFFKPGSQFLYSTFGYTLLAAIVERASGCKYLDYMQKIFHDLDMLTTVQEENEPVIYNRAR Predict reactive species\nFull Name: lactamase, beta\nCalculated Molecular Weight: 547 aa, 61 kDa\nObserved Molecular Weight: 50-60 kDa\nGenBank Accession Number: BC067288\nGene Symbol: LACTB\nGene ID (NCBI): 114294\nRRID: AB_2280998\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P83111\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868912930985,"sku":"18195-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-18195-1-ap","provider":"Iright","version":"1.0","type":"link"}