{"product_id":"proteintech-18980-1-ap","title":"Proteintech, 18980-1-AP, ZCCHC11 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ZCCHC11 (18980-1-AP) by Proteintech is a Polyclonal antibody targeting ZCCHC11 in WB, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n18980-1-AP targets ZCCHC11 in WB, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF7 cells,  HEK-293T cells,  HeLa cells,  human brain tissue\nPositive IP detected in: MCF-7 cells\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: zinc finger, CCHC domain containing 11\nCalculated Molecular Weight: 185 kDa\nObserved Molecular Weight: 185 kDa\nGenBank Accession Number: NM_015269\nGene Symbol: ZCCHC11\nGene ID (NCBI): 23318\nRRID: AB_10598327\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q5TAX3\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868868694185,"sku":"18980-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-18980-1-ap","provider":"Iright","version":"1.0","type":"link"}