{"product_id":"proteintech-18989-1-ap","title":"Proteintech, 18989-1-AP, GLI2-Specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GLI2-Specific (18989-1-AP) by Proteintech is a Polyclonal antibody targeting GLI2-Specific in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n18989-1-AP targets GLI2-Specific in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, HEK-293 cells,  human testis tissue\nPositive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells, MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, chamaeleo calyptratus\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: GLI family zinc finger 2\nCalculated Molecular Weight: 168 kDa\nObserved Molecular Weight: 167 kDa， 88 kDa\nGenBank Accession Number: NM_005270\nGene Symbol: GLI2\nGene ID (NCBI): 2736\nRRID: AB_10596479\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P10070\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868840054953,"sku":"18989-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-18989-1-ap","provider":"Iright","version":"1.0","type":"link"}